Abstract
The effect of feeding mycotoxins, i.e. aflatoxin B1 (1.25 ppm from 3 to 38 days of age) and ochratoxin A (0.5 ppm from 3 to 38 days of age) along with inclusion body hepatitis virus (IBHV) inoculation (at 10 days of age) singly and in combination was studied in broiler chicks. Birds in combined treatment groups, i.e. aflatoxin fed and virus inoculated and ochratoxin fed and virus inoculated, showed more changes in activities of phosphatases (AKPase, ATPase, G-6-Pase and ACPase) in liver and kidney tissues than their respective individual treatment groups with a few exceptions. Reduction in the activities of oxido-reductases in liver and kidney tissues were almost comparable in different treatment groups. The increase in muco-polysaccharides reaction was more marked in both the combined treatment groups than the single treatment groups. Intensity of lipid reaction was more in ochratoxin virus combination group than either alone.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Amer HA, Awad ET, El Batravi AM. Effect of aflatoxin B1 on alkaline phosphatase and lactate dehydrogenase levels in liver and serum of broiler chicks. Archives fur Experimentelle Veterinarmedizin 1988; 42: 595–602.
Smith JE, Moss MO. Mycotoxins-formation: Analysis and significance. New York: John Wiley and Sons, 1985.
Dwivedi P. The immunological and pathological changes in poultry induced by OA. PhD Thesis, University of Edinburgh, Scotland, 1984 (cited by Dwivedi P, Burns RB. World Poultry Sci J 1986; 42: 32–47).
Mishra SK. Studies on the prevalence and pathology of inclusion body hepatitis in broiler chicks. PhD Thesis, PAU, Ludhiana, India, 1988.
Reece RL, Grix DC, Barr DA. An unusual case of inclusion body hepatitis in cockrel. Avian Dis 1986; 30: 224–27.
Woernle H. The use of agar gel diffusion technique in identification of certain avian virus diseases. The Veterinarian 1966; 4: 17–28.
Oberoi MS, Ramneek, Maiti NK, Sharma SN. A rapid indirect dot enzyme immunoassay for direct detection of viruses. Indian J Comp Microbiol Immunol Infect Dis 1992 (in press).
Romer TR. Screening method for the detection of aflatoxin in mixed feed and other agricultural commodities with subsequent confirmation and quantitative measurement of aflatoxin in positive samples. J Assoc Off Anal Chem 1975; 58: 500–6.
Hald B, Krogh P. Detection of ochratoxin A in Barley, using silica gel minicolumns. J Assoc Off Anal Chem 1975; 58: 156–58.
Berka T, Anderson PJ. Histochemistry: Theory, Practice and Bibliography. New York: Hoeber Medical Division, Harper & Row, 1963.
Pearse AGE. Histochemistry Theoretical and Applied, 3rd ed. London: Churchill Livingstone 1968.
Chayen J, Bitensky L, Butcher RG, Poulter LW. A guide to practical histochemistry, 2nd edn. Edinburgh: Oliver & Boyd, 1973.
Raina JS, Roy KS and Singh B. Biochemical and histochemical studies in experimental mycotoxicosis in chicks. Indian J Anim Sci 1991; 62: 1276–81.
Sandhu BS. Studies on pathology of ochratoxin toxicity, inclusion body hepatitis and their interaction in poultry. MVSc Thesis PAU, Ludhiana, India, 1991.
Singh H. Studies on pathology of aflatoxicosis, inclusion body hepatitis and their interaction in poultry. MVSc Thesis, PAU, Ludhiana, India, 1991.
Theron, JJ. Acute liver injury in ducklings as a result of aflatoxin poisoning. Lab Invest 1965; 14: 1586–1603.
Brown JMM, Abrams I. Biochemical studies on aflatoxicosis. Onderstepoort J Vet Res 1965; 32: 119–45.
Warren F, Hamilton PB. Inhibition of the glycogen phosphorylase system during ochratoxicosis in chickens. Appl Environ Microbiol 1980; 40: 522–25.
Singh, AP. Epidemiological and experimental studies of inclusion body hepatitis in broiler chicks. MVSc Thesis, PAU, Ludhiana, India, 1990.
Krvavica S, Hadziosmanovic A, Francetic D, Bogdanic C. Effect of ochratoxin A + B on the activity of NADP + isocitrate dehydrogenase alphaketoglutaric dehydrogenase and succinate dehydrogenase in the kidneys and liver of fowls. Veterinarski Archiv 1984; 54: 125–31 (Abstract in: Rev Med Vet Mycol 1985; 20: 610).
Caranghan RBA, Herbert CN, Paterson DSP, Sweasey D. Comparative biological and biochemical study in hybrid chicks, 2: Susceptibility to aflatoxin and effect on serum protein constituents. Br Poult Sci 1967; 8: 279–81.
Reddy DN, Rao PV, Reddy VR, Yadgiri B. Effect of selected levels of dietary aflatoxin on the performance of broiler chicken. Indian J Anim Sci 1984; 54: 68–73.
Tucker TL, Hamilton PB. The effect of ochratoxin in broilers. Poult Sci 1971; 50: 1637.
Chu FS. Mode of action of mycotoxins and related compounds. In: Applied Microbiology. New York: Pearlman Academic Press, 1977: 83–147.
Meisner H, Ghan S. Ochratoxin A: an inhibitor of mitochondrial transport systems. Biochemistry 1974; 13: 2795–2800.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Singh, H., Singh, B., Roy, K.S. et al. Histochemical/histoenzymic studies in broiler chicks fed aflatoxin, ochratoxin and inoculated with inclusion body hepatitis virus singly and in concurrence. Mycopathologia 125, 119–128 (1994). https://doi.org/10.1007/BF01371100
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01371100