Summary
Haploid embryos and plantlets in yet another solanaceous member viz.,Capsicum annuum L. var.grossum Sendt, have been successfully rearedin vitro through culture of young immature anthers. Exogenous auxin (IAA) was essential for the initiation of cell division in pollen oriented towards a haploid morphogenesis. Of the several hundred anthers cultured, not more than 0.1% produced viable plantlets.
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References
Bourgin, J. P., etJ. P. Nitsch, 1967: Obtention deNicotiana haploïdes à partir d'étamines cultivéesin vitro. Ann. Physiol. Vég. (Paris)9, 377–382.
Debergh, P. M., etC. Nitsch, 1973: Premiers résultats sur la culturein vitro de graines de pollen isolés chez le Tomate. C. R. Acad. Sci. (Paris)276, 1281–1282.
Gresshoff, P. M., andC. H. Doy, 1972: Development and differentiation of haploidLycopersicon esculentum (tomato). Planta (Berl.)107, 161–170.
Guha, S., andS. C. Maheshwari, 1964:In vitro production of embryos from anthers ofDatura. Nature (London)204, 497.
Harn, C., 1971: Studies on anther culture inSolanum nigrum. SABRAO Newsletter3, 39–42.
Irikura, Y., andS. Sakaguchi, 1972: Induction of 12-chromosome plants from anther culture in a tuberousSolanum. Potato Res.15, 170–173.
Kohlenbach, H. W., undT. Geier, 1972: Embryonen ausin vitro kultivierten Antheren vonDatura metaloides Dun,D. wrightii Regal undSolanum tuberosum L. Z. Pflanzenphysiol.67, 161–163.
Linsmaier, E. M., andF. Skoog, 1965: Organic growth factor requirements of tobacco tissue cultures. Physiol. Plantarum18, 100–127.
Narayanaswamy, S., andL. P. Chandy, 1971:In vitro induction of haploid, diploid, and triploid androgenic embryoids and plantlets inDatura metel L. Ann. Bot.35, 535–542.
—, andL. George (néeChandy), 1972: Morphogenesis of belladonna (Atropa belladonna L.) plantlets from pollen in culture. Indian J. Exp. Biol.10, 382–384.
Raquin, C., etV. Pilet, 1972: Production de plantules à partir d'anthères dePétunias cultivéesin vitro. C. R. Acad. Sci. (Paris)274, 1019–1022.
Sunderland, N., 1971: Anther culture: a progress report. Sci. Prog. Oxf.59, 527–549.
—, andF. M. Wicks, 1971: Embryoid formation in pollen grains ofNicotiana tabacum. J. Exp. Biol.22, 213–226.
Zenkteler, M., 1971:In vitro production of haploid plants from pollen grains ofAtropa belladonna L. Experientia27, 1087.
—, 1972: Development of embryos and seedlings from pollen grains inLycium halimifolium in thein vitro culture. Biol. Plant.14, 420–422.
—, 1973:In vitro development of embryos and seedlings from pollen grains ofSolanum dulcamara. Z. Pflanzenphysiol.69, 189–190.
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George, L., Narayanaswamy, S. HaploidCapsicum through experimental androgenesis. Protoplasma 78, 467–470 (1973). https://doi.org/10.1007/BF01275781
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DOI: https://doi.org/10.1007/BF01275781