Abstract
Among opiatelike peptides, stimulatory as well as inhibitory effects are encountered on adenylate cyclase activity. These actions are dependent not only on the investigated brain region but also on the applied peptide. Met-enkephalin stimulates adenylate cyclase activity in the rat brain stem (d-Met2, Pro5)-enkephalinamide and β-endorphin inhibited it, whereas all three peptides inhibited the activity of cortex. Naloxone antagonized the effects of the applied peptides in the presence of sodium chloride.
Similar content being viewed by others
References
Collier, H. O. J., andRoy, A. C. 1974. Morphine-like drugs inhibit the stimulation by E prostaglandins of cyclic AMP formation by rat brain homogenate.Nature (London) 248:24–27.
Puri, S. K., Cochin, J., andVolicer, L. 1975. Effect of morphine sulfate on adenylate cyclase and phosphodiesterase activities in rat corpus striatum.Life Sci. 16:759–768.
Minneman, K. P., andIversen, L. L. 1976. Enkephalin and opiate narcotics increase cyclic GMP accumulation in slices of rat neostriatum.Nature (London) 262:313–314.
Sharma, S. K., Klee, W. A., andNirenberg, M. 1977. Opiate-dependent modulation of adenylate cyclase.Proc. Natl. Acad. Sci. USA 74:3365–3369.
Goldstein, A., Cox, B. M., Klee, W. A., andNirenberg, M. 1977. Endorphin from pituitary inhibits cyclic AMP formation in homogenates of neuroblastomaxglioma hybrid cells.Nature (London) 265:362–363.
Wollemann, M., Borbola, J., Papp, J. Gy., andSzekeres, L. 1975. Cardiac adenylate cyclase activity in relation to beta-adrenergic responses.J. Mol. Cell. Card. 7:523–533.
Lowry, O. H., Rosebrough, H. J., Farr, A. L., andRandall, R. J. 1951. Protein measurement with the Folin phenol reagent.J. Biol. Chem. 193:265–275.
Gráf, L., Barát, E., andPatthy, A. 1976. Isolation of a COOH-terminal β-lipotropin fragment (residues 61–91) with morphine-like analgesic activity from porcine pituitary glands.Acta Biochim. Biophys. Acad. Sci. Hung. 11:121–122.
Bajusz, S., Rónai, A. Z., Székely, J. I., Gráf, L., Dunai-Kovács, Zs., andBerzétei, I. 1977. A superactive antinociceptive pentapeptide, (d-Met2,Pro5)-enkephalinamide.FEBS Lett. 76:91–92.
Simon, E. J., andHiller, J. M. 1978.In vitro studies on opiate receptors and their ligands.Fed. Proc. 37:141–146.
Rossier, J., Vargo, T. M., Minick, S., Ling, N., Bloom, F. E., andGuillemin, R. 1977. Regional dissociation of β-endorphin and enkephalin contents in rat brain and pituitary.Proc. Natl. Acad. Sci. USA 74:5162–5165.
Watson, S. J., Richard, C. W., andBarchas, J. D. 1978. Adrenocorticotropin in rat brain: Immunocytochemical localization in cells and axons.Science 200:1180–1182.
Lord, J. A. H., Waterfield, A. A., Hughes, J., andKosterlitz, H. W. 1977. Endogenous opioid peptides: Multiple agonists and receptors.Nature (London) 267:495–499.
Rónai, A. Z., Gráf, L., Székely, J. I., Dunai-Kovács, Zs., andBajusz, S. 1977. Differential behaviour of LPH-(61-91)-peptide in different model systems: Comparison of the opioid activities of LPH-(61-91)-peptide and its fragments.FEBS Lett. 74:182–184.
Calderini, G., Consolazione, A., Garattini, S., andAlgeri, S. 1978. Different effects of methionine-enkephalin and (d-Ala2) methionine-enkephalin amide on the metabolism of dopamine and norepinephrine in rat brain: Fact or artifact?Brain Res. 146:392–399.
Székely, J. I., Dunai-Kovács, Zs., Miglécz, E., Rónai, A. Z., andBajusz, S. 1979.In vivo antagonism by naloxone of morphine, β-endorphine and a synthetic enkephalin analog.J. Pharmacol. Exp. Ther. (in press).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Wollemann, M., Szebeni, A., Bajusz, S. et al. Effect of met-enkephalin and (d-Met2,Pro5)-enkephalinamide on the adenylate cyclase activity of rat brain. Neurochem Res 4, 627–631 (1979). https://doi.org/10.1007/BF00964439
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00964439