Abstract
A microenzyme-linked immunosorbent assay system employing monoclonal antibody SNH3 was developed for the detection of sialosyl Lewisx antigen in stool extracts from 80 patients with colorectal cancer, 13 patients with colorectal non-malignant disorders and 90 normal subjects. Sialosyl Lewisx antigen was detected in 35% of stool extracts from cancer patients but only in 7.7% and 2.2% of those from non-malignant patients and normal subjects respectively. Hemoglobin in the same stool samples was detected in 37.5% of cancer patients, 15.4% of non-malignant patients and 5.6% of normal subjects. The appearance of sialosyl Lewisx antigen in stool was not necessary correlated with that of hemoglobin, and overall 61.3% of cancer patients were detected by the combination of the two assays. The combination assay was also impressive in early detection of colorectal cancer (Dukes' A, 52%; Dukes' B, 57.1%). Therefore, the assay for sialosyl Lewisx antigen in stool would be useful for detecting colorectal cancer.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Solter D, Knowles BB. Monoclonal antibody defining a stage-specific embryonic antigen (SSEA-1).Proc Natl Acad Sci USA 1978:75; 5565–9.
Gooi HC, Feizi T, Kapadia A, Knowles BB, Solter D, Evans MJ. Stage-specific embryonic antigen involves α1→3fucosylated type 2 blood group chains.Nature (Lond) 1981:292; 156–8.
Kannag R, Nudelman E, Levery SB, Hakomori S. A series of human erythrocyte glycosphingolipids reacting to the monoclonal antibody directed to developmentally regulated antigen, SSEA-1.J Biol Chem 1982;257; 14865–74.
Miyake M, Zenita K, Tanaka O, Okada Y, Kannagi R. Stage-specific expression of SSEA-1-related antigens in the develo** lung of human embryos and its relation to the distribution of these antigens in lung cancers.Cancer Res 1988:48; 7150–8.
Fukushima K, Hirota M, Terasaki PI, et al. Characterization of sialosyl Lewisx as a new tumor-associated antigen.Cancer Res 1984:44; 5279–85.
Adams EC, Layman KM. Immunochemical conformation of gastrointestinal bleeding.Ann Clin Lab Sci 1974:4; 343–9.
Songster CL, Barlow GH, Jarrett DD. Immunochemical detection of fecal occult blood.Cancer 1980:45; 1099–102.
Sidransky D, Tokino T, Hamilton SR, et al. Identification ofras oncogene mutations in the stool of patients with curable colorectal tumors.Science 1992:256; 102–5.
Itzkowitz SH, Yuan M, Fukushi Y, et al. Lewisx-and sialylated Lewisx-related antigen expression in human malignant and non-malignant colonic tissues.Cancer Res 1986:46; 2627–32.
Yuan M, Itzkowitz SH, Ferrell LD, et al. Expression of Lewisx and sialylated Lewisx antigens in human colorectal polyps.J Natl Cancer Inst 1987:78; 479–88.
Hoff SD, Matsushita Y, Ota DM, et al. Increased expression of sialyl-dimetric Lex antigen in liver metastases of human colorectal carcinoma.Cancer Res 1989:49; 6883–8.
Matsushita Y, Cleary KR, Ota DM, Hoff SD, Irimura T. Sialyl-dimetric Lewis-X antigen expressed on mucin-like glycoproteins in colorectal cancer metastases.Lab Invest 1990:63; 780–91.
Phillips ML, Nudelman ED, Gaeta FCA, et al. ELAM-1 mediates cell adhesion by recognition of a carbohydrate ligand, sialyl-Lex.Science 1990:250; 1130–2.
Jagirdar J, Thung SN, Shah KD, Nudelman E, Singhal A, Paronetto F. Expression of sialylated LewisX antigen in chronic and neoplastic liver disease.Arch Pathol Lab Med 1992:116; 643–8.
Kannagi R, Fukushi Y, Tachikawa T, et al. Quantitative and qualitative characterization of human cancer-associated serum glycoprotein antigens expressing fucosyl or sialyl-fucosyl type 2 chain polylactosamine.Cancer Res 1986:46; 2619–26.
Hakomori S. Aberrant glycosylation in cancer cell in cancer cell membranes as focused on glycolipid: overview and perspective.Cancer Res 1985:45; 2405–14.
Hakomori S. Tumor-associated glycolipid antigens, their metabolism and organization.Chem Phys Lipids 1986:42; 209–33.
Yonezawa S, Tachikawa T, Shin S, Sato E. Sialosyl-Tn antigen: its distribution in normal human tissues and expression in adenocarcinomas.Am J Clin Pathol 1992:98; 167–74.
Yazawa S, Madiyalakan R, Izawa H. Asao T, Furukawa K, Matta KL. Cancer-associated elevation of α(1→3)-L-fucosyltransferase activity in serum.Cancer 1988:62; 516–20.
Tachikawa T, Yazawa S, Asao T, Shin S, Yanaihara N. Novel method for quantifying α(1→3)-L-fucosyltransferase activity in serum.Clin Chem 1991:37; 2081–6.
Fujimoto S, Kitsukawa Y, Itoh K. Carcinoembryonic antigen (CEA) in gastric juice of feces as an aid in the diagnosis of gastrointestinal cancer.Ann Surg 1979:189; 34–8.
Stubbs RS, Nadokarni DM, Monsey DA. Faecal carcinoembryonic antigen in colorectal cancer patients.Gut 1986:27; 901–5.
Hahn DL. Feasibility of sigmoidoscopic screening for bowel cancer in a primary care setting.J Am Board Family Pract 1989:2; 25–9.
Yamagata Y, Shigeta K, Masuda T et al. Glycolipid-directed FH-6 monoclonal antibody recognizes high molecular weight glycoproteins antigen carrying sialyl Lex-i determinant in the culture supernatant of PC-9 cells.J Mol Recog 1988:1; 111–15.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Tachikawa, T., Kamikawa, K., Chia, D. et al. Cancer-associated quantitative differences in antigen levels of sialosyl Lewisx in stool from patients with colorectal carcinomas. Glycosylation & Disease 1, 111–117 (1994). https://doi.org/10.1007/BF00919297
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF00919297