Abstract
In a field experiment, the effect of animal slurry, (with and without the nitrification inhibitor dicyandiamide on total denitrification losses estimated by the C2H2 inhibition technique was measured over 2 years (1989–1990). During this period, four different plots (each with four replicates) were fertilized six times with 150 kg N ha-1 in the form of cattle-pig slurry or NH4NO3. Soil samples (0–20 cm) were analysed at regular intervals for NH sup+inf4 and NO sup−inf3 concentrations. The soil water content was determined gravimetrically. During the first year (1989) total denitrification losses from unfertilized, mineral-fertilized, and animal slurry-amended plots (with or without dicyandiamide) were estimated as 0.2, 3.1, 0.7, and 0.6 kg N ha-1, respectively. During the second year (1990) the denitrification losses were 0.4, 1.3, 0.7, and 0.7 kg N ha-1, respectively. There was a clear relationship between the NO sup−inf3 concentration or soil water content and the denitrification rate. The results are siteund experiment-specific and cannot be generalized so far.
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Abou-Seada MNI, Ottow JCG (1985) Effect of increasing oxygen concentration on total denitrification and nitrous oxide release from soil by different bacteria. Biol Fertil Soils 1:31–38
Amberger A, Vilsmeier K, Gutser R (1982) Stickstoffreaktionen verschiedener Güllen und deren Wirkung im Pflanzenversuch. Z Pflanzenernaehr Bodenkd 145:325–336
Benckiser G, Haider K, Sauerbeck D (1986) Field measurements of gaseous nitrogen losses from an Alfisol planted with sugar-beets. Z Pflanzenernaehr Bodenkd 149:249–261
Benckiser G, Gaus G, Syring KM, Haider K, Sauerbeck D (1987) Denitrification losses from an Inceptisol field treated with mineral fertilizer or sewage sludge. Z Pflanzenernaehr Bodenkd 150:241–248
Crawford DM, Chalk PM (1992) Mineralization and immobilization of soil and fertilizer nitrogen with nitrification inhibitors and solvents. Soil Biol Biochem 24:559–568
Groffman PM, Tiedje JM (1989) Denitrification in north temperate forest soils: Relationship between denitrification and environmental factors at the landscape scale. Soil Biol Biochem 21:621–626
Guirauld G, Marol V, Fardeau JC (1992) Balance and immobilization of (15NH4)2SO4 in a soil after the addition of DIDIN as a nitrification inhibitor. Biol Fertil Soils 14:23–29
Haider K, Heinemeyer O, Mosier AR (1990) Direct and indirect effects of plants on denitrification. Mitt Dtsch Bodenkd Ges 60:101–108
Hoffmann G, Teicher K (1961) Ein kolorimetrisches Verfahren zur Bestimmung der Ureaseaktivität in Böden. Z Pflanzenernaehr Bodenkd 95:55–63
Kapp M, Schwarz J, Benckiser G, Ottow JCG, Daniel P, Opitz von Boberfeld W (1990) Der Einsatz der Acetylen-Inhibierungstechnik zur Quantifizierung von Denitrifikationsverlusten in unterschiedlich gedüngten Weidelgrasbeständen. Forum Städte-Hyg 41:168–172
Lehn-Reiser M, Munch JC, Chapot JY, Ottow JCG (1990) In-situ Messungen von Denitrifikationsverlusten einer kalkhaltigen Braunerde nach Einarbeitung verschiedener Gründüngungspflanzen (Vicia sativa, Brassica napus). Forum Städte-Hyg 41:164–167
McCarty GW, Bremner JM (1989) Inhibition of nitrification in soil by heterocyclic nitrogen compounds. Biol Fertil Soils 8:204–211
Moraghan JG, Buresh RJ (1977) Correction for dissolved nitrous oxide in nitrogen studies. Soil Sci Soc Am J 41:1201–1203
Mosier AR, Parton W (1985) Denitrification in a shortgrass prairie: A modeling approach. In: Caldwell DE, Brierly JA, Brierly CL (eds) Planetary ecology. Van Nostrand Reinhold, New York, pp 441–452
Mosier AR, Guenzi WD, Schweitzer EE (1986) Field denitrification estimation by nitrogen-15 and acetylene inhibition techniques. Soil Sci Soc Am J 50:831–833
Navone R (1964) Proposed method for nitrate in potable waters. J Am Water Works Assoc 56:781–783
Ottow JCG (1992) Denitrifikation eine kalkulierbare Größe in der Stickstoffbilanz von Böden? Wasser und Boden 9:578–581
Ottow JCG, Fabig W (1985) Influence of oxygen on denitrifcation and redox level in different bacterial cultures. In: Caldwell DE, Brierly JA, Brierly CL (eds) Planetary ecology. Van Nostrand Reinhold, New York, pp 427–440
Parkin TB (1990) Characterizing the variability of soil denitrification. In: Revsbech NP, Soerensen J (eds) Denitrification in soil and sediment. Plenum Press, New York London, pp 213–228
Prade K, Trolldenier G (1988) Effect of wheat roots on denitrification at varying soil air-filled porosity and organic-carbon content. Biol Fertil Soils 7:1–6
Rajbanshi SS, Benckiser G, Ottow JCG (1992a) Mineralization kinetics and utilization as an N source of dicyandiamide (DCD) in soil. Naturwissenschaften 79:26–27
Rajbanshi SS, Benckiser G, Ottow JCG (1992b) Effects of concentration, incubation temperature and repeated application on degradation kinetics of dicyandiamide (DCD) in model experiments with a silt loam soil. Biol Fertil Soils 13:61–64
von Rheinbaben W (1990) Nitrogen losses from agricultural denitrification — a critical evaluation. Z Pflanzenernaehr Bodenkd 153:157–166
Ritzl I, Reiml D (1988) aufige und wichtige Folgen vermehrten Gülleanfalls. Z Wasser Abwasser Forsch 21:40–44
Ryden JC, Dawson KP (1982) Evolution of the acetylene inhibition technique for measurement of denitrification in grassland soils. J Sci Food Agric 33:1197–1206
Sahrawat KL, Keeney DR, Adams SS (1987) Ability of nitrapyrin, dicyandiamide and acetylene to retard nitrification in a mineral and an organic soil. Plant and Soil 101:179–182
Scharpf HC, Wehrman J (1976) Die Bedeutung des Mineralstickstoffvorrates des Bodens zu Vegetationsbeginn für die Bemessung der N-Düngung zu Winterweizen. Landwirtsch Forsch 32:100–114
Simarmata T, Benckiser G, Ottow JCG (1993) Effect of an increasing carbon-nitrate-N ratio on the reliability of acetylene in blocking the N2O-reductase activity of denitrifying bacteria in soil. Biol Fertil Soils 15:107–112
Thompson RB (1989) Denitrification in slurry-treated soil: Occurence at low temperatures, relationship with soil nitrate and reduction by nitrification inhibitor. Soil Biol Biochem 21:875–882
Vinther FP (1992) Measured and simulated denitrification activity in a cropped sandy and loamy soil. Biol Fertil Soils 14:43–48
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Schwarz, L., Kapp, M., Benckiser, G. et al. Evaluation of denitrification losses by the acetylene inhibition technique in a permanent ryegrass field (Lolium perenne L.) fertilized with animal slurry or ammonium nitrate. Biol Fert Soils 18, 327–333 (1994). https://doi.org/10.1007/BF00570636
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DOI: https://doi.org/10.1007/BF00570636