Summary
Up to now quantitative analysis of human bone enzymes is a part of osteology which is not evaluated sufficiently. The lack of standardized methods, fresh bone and reliable methods of bone disintegration seem to impede these experiments mainly. The authors have elaborated a complex method for quantitative measurements of human bone enzymes and they describe the practical use of this method. The importance of quantitative bone enzyme measurements is pointed out.
Zusammenfassung
Die quantitative Analyse der Enzyme menschlicher Knochen ist ein noch nicht ausreichend studierter Teil der Osteologie. Das Fehlen von standardisiertem frischem Knochenmaterial und eine bewährte Methode der Knochenzersetzung sind die Haupthindernissse, solche Versuche durchführen zu können. Die Verfasser arbeiteten eine komplexe Methode für Messungen der Enzyme menschlicher Knochen aus. Die Methode and deren Probe wird beschrieben. Die Wichtigkeit der quantitativen Messungen menschlicher Knochenenzyme wird betont.
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References
Bergmeyer HU (1963) Methods of enzymatic analysis. Academic Press New York London, pp. 736–740
Hansen PW (1966) Simplification of Kind and King's method for determination of serum phosphatase. Scand J Clin Lab Invest 18:353–356
Jeffree GM, Price CH (1965) Bone tumours and their enzymes. J Bone Jt Surg 478:120–136
Lereim P, Linde A, Goldie JF (1975) The presence of alkaline phosphatase in the subchondral bone of the medial tibial condyle in the normal state and in osteoarthritis and rheumatoid arthritis. Arch Orthop Unfall Chir 83:181–185
Lénárt G, Széll V, Csorba E (1971) Comparative investigations on bone and callus enzymes. Acta Biochim Biophys Acad Sci Hung 6:243–250
Lowry OH, Rosebrough NI, Farr AL, Randall RJ (1951) Protein measurement with the folin phenol reagent. J Biol Chem 193:265–275
Semb TH (1970) Isozymes of bone esterases. Preliminary report. Calc Tiss Res 6:77–80
Semb H (1971) Isoenzymes of lactic dchydrogenase in bone and bone marrow plasma. Clin Orthop 79:205–209
Vaes G, Jacques P (1965) Studies on bone enzymes. Biochim J 97:380–402
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Lénárt, G., Széll, V. Quantitative measurements of human bone enzymes. Arch. Orth. Traum. Surg. 97, 103–105 (1980). https://doi.org/10.1007/BF00450931
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DOI: https://doi.org/10.1007/BF00450931