Summary
In Aspergillus nidulans there are two alcohol dehydrogenases. In the presence of ethanol, alcohol dehydrogenase I (AHH I) is induced and alcohol dehydrogenase II (ADH II) is repressed. ADH I and ADH II have molecular weights of 39,000 and 36,000 respectively. At least ADH I is under the control of alcR, a transacting regulatory gene that is adjacent to alcA (the structural gene for ADH I, Pateman et al. 1983). Mutations in the alcR regulatory gene result in non inducibility of ADH I specific mRNA. Extreme alcA and alcR mutations result in derepressed levels of ADH II, and it is not clear whether alcR controls ADH II directly or through its control of ADH I synthesis. Both enzymes are subject to carbon catabolite repression. Induction of ADH I and ADH II operates at the level of synthesis or processing of mRNA.
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References
Beier DR, Young ET (1982) Nature 300:724–728
Bennetzen JL, Hall BD (1982) Biol Chem 257:3018–3025
Benyati C, Place AR, Powers DA, Sofer W (1981) Proc Natl Acad Sci USA 78:2717–2721
Bonner WM, Laskey RA (1974) Eur J Biochem 46:83–88
Ciriacy M (1975a) Mutat Res 25:315–326
Ciriacy M (1975b) Mol Gen Genet 138:157–164
Ciriacy M (1976) Mol Gen Genet 145:327–333
Cove DJ (1966) Biochem Biophys Acta 113:51–56
Cox RA (1978) Methods Enzymol 12B:120–129
Deeley RG, Gordon J, Burns AJH, Mullinix KP, Binastein M, Goldberger RF (1977) J Biol Chem 252:8310–8319
Dottavia-Martin D, Ravel JM (1978) Anal Biochem 87:562–565
Hynes MJ, Corrick CM, King JA (1983) Mol Cellular Biol (In press)
Jackson RJ, Hunt T (1983) Methods Enzymol 96:50–74
Kessler SW (1975) A J Immunol 115:1617–1624
Laemmli UK (1970) Nature 227:680–685
Lewis NJ, Gilbert J (1978) Biochem Genet 16:159–170
Li Ti-K, Bosron WF, Dateldecker WP, Lange LG, Vallee BL (1977) Proc Natl Acad Sci USA 74:4378–4381
Lutstorf U, Megnet R (1968) Arch Biochem Biophys 126:933–944
McCully KS, Forbes E (1965) Genet Res 6:352–359
McDonald JF, Chambers GKL, David J, Ayala FJ (1977) Proc Natl Acad Sci USA 74:4378–4381
Page MM (1971) PhD thesis, University of Cambridge
Pateman JA, Doy CH, Olsen JE, Norris U, Creaser EH, Hynes M (1983) Proc R Soc Lond B217:243–264
Pelham HRB, Jackson RJ (1976) Eur J Biochem 67:247–256
Roberts TJ, Martinelli S, Scazzocchio C (1979) Mol Gen Genet 177:57–64
Pontecorvo G, Roper JA, Hemmons LM, MacDonald KD, Bufton AWJ (1953) Adv Genet 5:141–238
Russell DW, Smith M, Williamson VM, Young ET (1983) J Biol Chem 258:2674–2682
Scazzocchio C, Holl FB, Foguelman AI (1973) Eur J Biochem 36:428–445
Schwarz D (1976) Proc Natl Acad Sci USA 73:582–584
Smith M, Hopkinson DA, Harris H (1973) Ann Hum Genet 37:1929–1933.
Thorley-Lawson DA (1979) Cell 16:33–42
Williamson VM, Bennetzen J, Young ET, Nasmyth K, Hall BD (1980) Nature 283:214–216
Williamson VM, Young ET, Ciriacy M (1981) Cell 23:605–614
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Sealy-Lewis, H.M., Lockington, R.A. Regulation of two alcohol dehydrogenases in Aspergillus nidulans . Curr Genet 8, 253–259 (1984). https://doi.org/10.1007/BF00419721
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DOI: https://doi.org/10.1007/BF00419721