Abstract
A new iso-accepting tRNAphe from extracts of chemoheterotrophic and photoheterotrophic cells of Rhodopseudomonas sphaeroides has been identified by both BDEAE cellulose and RPC-5 chromatography. Rechromatography of each of the tRNAphe species in either the acylated or deacylated state shows that they migrate as single homogeneous peaks.
In steady-state chemoheterotrophic cultures of R. sphaeroides tRNA pheI–II account for 25–30% of the total phenylalanine accepting activity while in steadystate photoheterotrophic cultures tRNA pheI–II account for no more than 10% of the total phenylalanine accepting activity.
During the transition from chemoheterotrophic to photoheterotrophic growth conditions the levels of tRNA pheI–II fall in an exponential manner during the first half of the intracytoplasmic membrane induction period. tRNA pheI then remains at a level 10% that of its steady-state chemoheterotrophic level as long as photoheterotrophic growth conditions remain. tRNA pheII , after drop** to 10% of its former chemoheterotrophic level then returns to a level 50% that of its chemoheterotrophic level as long as photoheterotrophic growth conditions remain.
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Abbreviations
- BDEAE:
-
benzoylated diethyl amino ethyl
- RPC:
-
reversed phase chromatography
- TCA:
-
tricholroacetic acid
- ICM:
-
intracytoplasmic membrane
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Submitted by WDS in partial fullfilment of requirements for the M.S. degree
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Shepherd, W.D., Kaplan, S. Changes in Rhodopseudomonas sphaeroides isoaccepting phenylalanyl-tRNA species during transitions from chemoheterotrophic to photoheterotrophic growth. Arch. Microbiol. 116, 161–167 (1978). https://doi.org/10.1007/BF00406031
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DOI: https://doi.org/10.1007/BF00406031