Abstract
A freshly-prepared yeast extract at 30 or 50 g/l improved the growth of Bradyrhizobium japonicum SEMIA 587 in a 5-l stirred fermenter. Monosodium glutamate or a commercial yeast extract at 2.0 g/l almost doubled cell mass productivity and cell viability when added at the end of the first exponential growth phase.
References
American Oil Chemists' Society 1982 Glycerol. In Official and tentative Methods of Analysis, 3rd edn, pp. 3–5. Champaign, IL: AOCS.
Bonomi, A. 1986 Technological development for the industrial production of Rhizobium japonicum. Anais da Academia de Ciências do Estado de São Paulo 51, 209–305.
Lopreto, C., Mazza, L.A. & Ballati, A.P. 1972 Influencia de los componentes del medio de cultivo sobre el tiempo de generacion de una cepa de Rhizobium japonicum. Anales de la Sociedad Cientifica Argentina 193, 35–47.
Mazza, L.A., Lopreto, C. & Ballati, A.P. 1976 Obtencion de cultivos de Rhizobium japonicum en un fermentador sin agitacion mecanica. Revista Associacion Argentina de Microbiologia 8, 99–103.
Miles, A. & Misra, S. 1938 The estimation of the bactericidal power of the blood. Journal of Hygiene 38, 732–749.
Additional information
The authors are with the Divisão de Quimica, Agrupamento de Biotecnologia, Instituto de Pesquisas Tecnológicas do Estado de São Paulo, S/A.-IPT-Cidade Universitária s/n., Caixa Postal 7141, CEP 01064-970, São Paulo, SP, Brazil
Rights and permissions
About this article
Cite this article
Pradella, J.G.C., Oliveira, M.S., Zuccolo, M. et al. Improved culture media for growth of Bradyrhizobium japonicum . World Journal of Microbiology & Biotechnology 10, 112–113 (1994). https://doi.org/10.1007/BF00357575
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00357575