Abstract
We analyzed by scanning electron microscopy (SEM) the intact lampbrush chromosomes of the Pleurodeles oocyte karyotype. Each of the 12 bivalents was identified and photomicrographed in phase contrast and at low SEM magnification before the structure of chromomeres and lateral loops was analyzed at higher magnifications. Stereographic examination showed that the chromomere structure is not as well defined as it appears in the light microscope. The characteristic morphology of typical landmarks such as granular or globular loops seems to be due to a more or less tight packing and coiling of matrix components. This packing probably corresponds to the storage of transcription products and therefore of genic information.
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References
Angelier N, Lacroix JC (1975) Complexes de transcription d'origines nucléolaire et chromosomique d'ovocytes de Pleurodeles waltlii et P. poireti. Chromosoma 51:323–335
Angelier N, Lavaud A (1982) Improvements in the ultrastructural approach of lampbrush chromosomes of amphibians. IX. Congress of the ISDB, Basel, Swizerland, August 1981. In: Burger A, Weber MM (eds) Embryonic development. 85A: Genetic Aspects; Alan Liss, Inc., New York, pp 187–198
Callan HG (1963) The nature of lampbrush chromosomes. Int Rev Cytol 15:1–34
Callan HG, Lloyd L (1960) Lampbrush chromosomes of crested newts Triturus cristatus Laurenti. Philos Trans R Soc B 243:135–219
Daskal Y, Mace ML Jr, Wray W, Busch H (1976) Use of direct current sputtering for improved visualization of chromosome topology by scanning electron microscopy. Exp Cell Res 100:204–212
Daskal Y, Ballal NR, Busch H (1978a) Ultrastructural and biochemical studies on the isolation of nucleolar chromatin from Novikoff hepatoma cell nucleoli. Exp Cell Res 111:153–165
Daskal Y, Mace ML Jr, Busch H (1978b) Demonstration of membranous patches on isolated chromosomes. Exp Cell Res 111:472–475
Gall JG (1954) Lampbrush chromosomes from oocyte nuclei of the newt. J Morphol 94:283–351
Gall JG, Callan HG (1962) 3H-uridine incorporation in lampbrush chromosome. Proc Natl Acad Sci 48:562–570
Lacroix JC (1968) Étude descriptive des chromosomes en écouvillon dans le genre Pleurodeles (Amphibien, Urodèle). Ann Embryol Morphol 1:179–202
Malcolm DB, Sommerville J (1974) The structure of chromosomederived ribonucleoprotein in oocytes of Triturus cristatus carnifex (Laurenti). Chromosoma 48:137–158
Malcolm DB, Sommerville J (1977) The structure of nuclear ribonucleo-protein of amphibian oocytes. J Cell Sci 24:143–165
Mancino G, Barsacchi G (1965) Le mappe dei cromosomi “lampbrush” di Triturus (Anfibi, Urodeli). I. Triturus alpestris apuanus, Caryologia 18:637–665
Mancino G, Barsacchi G (1966) Le mappe dei cromosomi “lampbrush” di Triturus (Anfibi, Urodeli). II. Triturus helveticus helveticus. Riv Biol 19:311–351
Miller OL, Beatty BR (1969) Visualization of nucleolar genes. Science 164:955–957
Mott MR, Callan HG (1975) An electron microscope study of the lampbrush chromosomes of the newt Triturus cristatus. J Cell Sci 17:241–261
Scheer U, Trendelenburg MF, Franke WW (1973) Transcription of ribosomal cistrons. Correlation of morphological and biochemical data. Exp Cell Res 80:175–190
Scheer U, Franke WW, Trendelenburg M, Spring H (1976) Classification of loops of lampbrush chromosome according to the arrangement of transcriptional complexes. J Cell Sci 22:503–519
Scheer U, Spring H, Trendelenburg MF (1979) Organization of transcriptionally active chromatin in lampbrush chromosome loops. In: Busch H (ed) Cell nucleus, vol. VII. Academic Press, New York, pp 4–44
Skoglund U, Anderson K, Björkroth B, Lamb MM, Daneholt B (1983) Visualization of the formation of transport of a specific hnRNP. Cell 34:847–855
Snow MHL, Callan HG (1969) Evidence for a polarized movement of the lateral loops of newt lampbrush chromosomes during oogenesis. J Cell Sci 5:1–25
Sommerville J (1981) Immunolocalization and structural organization in nascent RNP. In: Busch H (ed) Cell nucleus, vol. VIII. Academic Press, New York, pp 1–52
Sommerville J, Malcolm DB (1976) Transcription of genetic information in amphibian oocytes. Chromosoma 55:183–208
Spring H, Franke WW (1981) Transcriptionally active chromatin in loops of lampbrush chromosomes at physiological salt concentrations as revealed by electron microscopy sections. Eur J Cell Biol 24:298–308
Utsumi KR (1982) Scanning electron microscopy of Giemsastained chromosomes and surface spread chromosomes. Chromosoma 86:683–702
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Angelier, N., Paintrand, M., Lavaud, A. et al. Scanning electron microscopy of amphibian lampbrush chromosomes. Chromosoma 89, 243–253 (1984). https://doi.org/10.1007/BF00292471
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DOI: https://doi.org/10.1007/BF00292471