Summary
Two experiments were carried out on a dysmoder humus sampled from a depth of 2–5 cm from a mixed hardwood forest. In acid soil, the chloroform fumigation-incubation method failed to estimate the microbial biomass, not because bacterial growth was inhibited after fumigation but because a labile C source was taken up which differed from the killed biomass C.
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Coûteaux, M.M., Henkinet, R., Pitta, P. et al. Native carbon mineralization of an acid organic soil after use of the chloroform-fumigation method to estimate microbial biomass. Biol Fert Soils 8, 172–177 (1989). https://doi.org/10.1007/BF00257762
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DOI: https://doi.org/10.1007/BF00257762