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Isolation of plasma membrane fragments and vesicles from ascites fluid of lymphoma-bearing mice and their possible role in the escape mechanism of tumors from host immune rejection

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Summary

Plasma membranes, generated in vivo by actively growing YAC lymphoma cells, were isolated from cell-free ascites fluid of lymphoma-bearing mice. Partial purification of the ascites fluid (AF) by means of ultracentrifugation resulted in the identification of two main fractions: (a) membrane fragments (AFM s ) and (b) membrane vesicles (AFM p ). Electron microscopy studies, polyacrylamide gel electrophoresis, marker enzymes, and binding capacity of radioactive lectins, have indicated that these membranes are released from the cell surface of YAC lymphoma cells, presumably by a shedding-off mechanism.

In vitro studies have demonstrated that the isolated membranes can specifically inhibit the association of normal macrophages and YAC lymphoma cells. In vivo studies have shown that these membranes can immunize against YAC tumors if injected intramuscularly or subcutaneously into adult mice. The results indicate that the ascites fluid membranes bear tumor-specific antigenic determinants.

Our results suggest that in vivo shedding of plasma membrane fragments or of membrane vesicles by actively growing YAC lymphoma cells may induce a self-protection of ascites tumors from host immune rejection.

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Abbreviations

YAC=:

Moloney-virus-induced lymphoma cells grown in A-strain mice

AF=:

ascites fluid of YAC lymphoma-bearing mice

AFMs and AFMp=:

membrane fragments and vesicles isolated from AF

PBS=:

phosphate-buffered saline

Con A=:

Concanavalin A

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Author notes

  1. A. Raz

    Present address: Frederik Cancer Research Center, Frederik, Maryland, USA

Authors and Affiliations

  1. Department of Membrane Research, The Weizmann Institute of Science, Rehovot, Israel

    A. Raz, R. Goldman, I. Yuli & M. Inbar

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  1. A. Raz
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  2. R. Goldman
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  3. I. Yuli
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  4. M. Inbar
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Raz, A., Goldman, R., Yuli, I. et al. Isolation of plasma membrane fragments and vesicles from ascites fluid of lymphoma-bearing mice and their possible role in the escape mechanism of tumors from host immune rejection. Cancer Immunol Immunother 4, 53–59 (1978). https://doi.org/10.1007/BF00205571

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  • DOI: https://doi.org/10.1007/BF00205571

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