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The function of the octamer-binding site in the DRA promoter

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Abstract

The octamer binding site, which is located immediately upstream of the poorly conserved DRA TATA sequence, is important fof high levels of expression of this human major histocompatibility class II gene in B cells. In this study, we demonstrate that the substitution of the DRA TATA sequence with the TATA box from the adenovirus E1b promoter revomes the requirements for the octamer binding site for high levels of expression from DRA promoter. Since only the TATA box from the E1b but bot the DRA promoters binds the TATA binding protein, we conclude that the octamer binding site helps to recruit TBP to the DRA promoter.

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References

  • Ausubel, F. M., Brent, R., Kingston, R. E., Moore, D. D., Seidman, J. G., Smith, J. A., and Struhl, K. Current Protocols in Molecular Biology, J. Wiley and Sons, New York, 1992

    Google Scholar 

  • Azizkan, J. C., Jensen, D. E., Pierce, A. J., and Wade, M. Transcription from TATA-less promoters: dihidrofolate reductase as model. Crit Rev Eukaryot Gene Expr 3: 229–254, 1993

    Google Scholar 

  • Benoist, C. and Mathis, D. Regulation of MHC class II gene expression: X, Y and other letters of the alphabet. Annu Rev Immuno 8: 681–715, 1990

    Google Scholar 

  • Corcoran, L., Karvelas, M., Nossal,. J., Ye, Z. S., Jacks, T., and Baltimore, D. Oct-2, although not required for early B-cell development, is critical for later B-cell maturation and for postnatal survival. Genes Dev 7: 570–582, 1993

    Google Scholar 

  • De Wet, J. R., Wood, K. V., DeLuca, M., Helinski, D. R., and Subramani, S. Firefly luciferase gene: structure and expression in mammalian cells. Mol Cell Biol 7: 725–737, 1987

    Google Scholar 

  • Dedrick, R. L. and Jones, P. P. Sequence elements required for activity of a murine major histocompatibility complex class II promoter bind common and cell-type-specific nuclear factors. Mol Cell Biol 10: 593–604, 1990

    Google Scholar 

  • Du, H., Roy, A. L., and Roeder, R. G. Human transcription factor USF stimulates transcription through the initiator elements of the HIV-1 and the Ad-ML promoters. EMBO J 12: 501–511, 1993

    Google Scholar 

  • Eisinger, D. P. and Serrero, G. A simple method for obtaining adaptors for routine subcloning. Biotechniques 15: 219, 1993

    Google Scholar 

  • Glimcher, L. H. and Kara, C. J. Sequences and factors: a guide to MHC class II transcription. Annu Rev Immunol 10: 13–49, 1992

    Google Scholar 

  • Gstaiger, M., Knoepfel, L., Georgiev, O., Schaffner, W., and Hovens, C. M. A B-cell coactivator of octamer-binding transcription factors. Nature 373: 360–362, 1995

    Google Scholar 

  • Hernandez, N. TBP, a universal eucaryotic transcription factor. Genes Dev 7: 1291–1308, 1993

    Google Scholar 

  • Herr, W., Sturm, R. A., Clerc, R. G., Corcoran, L. M., Baltimore, D., Sharp, P. A., Ingraham, H. A., Rosenfeld, M. G., Finney, M., Ruvkun, G., and Horvitz, H. R. The POU domain: a large conserved region in the mammalian pit-1, oct-1, oct-2, and Caenorhabditis elegans unc-86 gene products. Genes Dev 2: 1513–1516, 1988

    Google Scholar 

  • Kao, C. C., Lieberman, P. M., Schmidt, M. C., Zhou, Q., Pei, R., and Berk, A. J. Cloning of a transcriptionally active human TATA binding factor. Science 248: 1646–1650, 1990

    Google Scholar 

  • Kemler, I. and Schaffner, W. Octamer transcription factors and the cell type-specificity of immunoglobulin gene expression. FASEB J 4: 1444–1449, 1993

    Google Scholar 

  • Lanier, L. L. and Warner, N. L. Cell cycle related heterogeneity of Ia antigen expression on a murine B lymphoma cell line: analysis by flow cytometry. J Immunol 126: 626–631, 1981

    Google Scholar 

  • Lillie, J. W. and Green, M. R. Transcription activation by the adenovirus E1a protein. Nature 338: 39–44, 1989

    Google Scholar 

  • Long, E. O., Rosen-Bronson, S., Karp, D. R., Malnari, M., and Sekaly, R. Efficient cDNA expression vectors for stable and transient expression of HLA-DR in transfected fibroblast and lymphoid cells. Hum Immunol 31: 229–235, 1991

    Google Scholar 

  • Mantovani, R., Tora, L., Moncolin, V., Egly, J. M., Benoist, C., and Mathis, D. The major histocompatility complex (MHC) Ea promoter: sequences and factors at the initiation site. Nucleic Acids Res 21: 4873–4878, 1993

    Google Scholar 

  • Matsushima, G. K., Toh-Lindstrom, Y., and Ting, J. P.-Y Activation of the HLA-DRA gene in primary human T lymphocytes: novel usage of TATA and the X and Y promoter elements. Mol Cell Biol 12: 5610–5619, 1992

    Google Scholar 

  • Sambrook, J., Fritsch, E. F., and Maniatis, T. Molecular Cloning: A Laboratory Manual, 2nd edn, Cold Spring Harbor Laboratory, Cold Spring Harbor, 1989

    Google Scholar 

  • Sherman, P. A., Basta, P. V., Heguy, A., Wloch, M. K., Roeder, R. G., and Ting, J. P.-Y. The octamer motif is a B-lymphocyte-specific regulatory element of the HLA-DR alpha gene promoter. Proc Natl Acad Sci U S A 86: 6739–6743, 1989

    Google Scholar 

  • Stimac, E., Lyons, S., and Pious, D. Transcription of HLA class II genes in the absence of B-cell-specific octamer-binding factor. Mol Cell Biol 8: 3734–3739, 1988

    Google Scholar 

  • Strubin, M., Newell, J. W., and Matthias, P. OBF-1, a novel B cell-specific coactivator that stimulates immunoglobulin promoter activity through association with octamer-binding proteins. Cell 80: 497–506, 1995

    Google Scholar 

  • Ting, J. P.-Y. and Baldwin, A. Regulation of MHC gene expression. Curr Opin Immunol 5: 8–16, 1993

    Google Scholar 

  • Tsang, S. Y., Nakanishi, M., and Peterlin, B. M. B cell-specific and interferon-γ-inducible regulation of the HLA-DRa gene. Proc Natl Acad Sci USA 85: 8598–8602, 1988

    Google Scholar 

  • Tsang, S. Y., Nakanishi, M., and Peterlin, B. M. Mutational analysis of the DRA promoter: cis-acting sequences and trans-acting factors. Mol Cell Biol 10: 711–719, 1990

    Google Scholar 

  • Viville, S., Jongeneel, V., Koch, W., Mantovani, R., Benoist, C., and Mathis, D. The Eα promoter: a linker-scanning analysis. J Immunol 146: 3211–3217, 1991

    Google Scholar 

  • Voliva, C. F., Aronhein, A., Walker, M. D., and Peterlin, B. M. B-cell factor 1 is required for optimal expression of the DRA promoter in B cells. Mol Cell Biol 12: 2383–2390, 1992

    Google Scholar 

  • Voliva, C. F., Tsang, S., and Peterlin, B. M. Map** cis-acting defects in promoters of transcriptionally silent DQA2, DQB2, and DOB genes. Proc Natl Acad Sci USA 90: 3408–3412, 1993

    Google Scholar 

  • Wirth, T., Staudt, L., and Baltimore, C. An octamer oligonucleotide upstream of a TATA motif is sufficient for lymphoid specific promoter activity. Nature 329: 174–178, 1987

    Google Scholar 

  • Wobbe, C. R. and Struhl, K. Yeast and human TATA-binding proteins have nearly identical DNA sequence requirements for transcription in vitro. Mol Cell Biol 10: 3859–3867, 1990

    Google Scholar 

  • Zeleznik-Le, N. J., Itoh-Lindstrom Y., Clarke, J. B., Moore, T. L., and Ting, J. P.-Y. The B cell-specific nuclear factor OTF-2 positively regulates transcription of the human class II transplantation gene, DRA. J Biol Chem 267: 7677–7682, 1992

    Google Scholar 

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Authors and Affiliations

  1. Searle Research and Development, Monsanto Company, 700 Chesterfield Village Parkway, Mail Zone AA4C, 63198, St. Louis, MO, USA

    Charles F. Voliva

  2. Departments of Medicine, Microbiology and Immunology, University of California, San Francisco, Howard Hughes Medical Institute, 3rd and Parnassus Avenues, Box 0724, 94143, San Francisco, CA, USA

    Nabila Jabrane-Ferrat & B. Matija Peterlin

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  1. Charles F. Voliva
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  2. Nabila Jabrane-Ferrat
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  3. B. Matija Peterlin
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Voliva, C.F., Jabrane-Ferrat, N. & Matija Peterlin, B. The function of the octamer-binding site in the DRA promoter. Immunogenetics 43, 20–26 (1995). https://doi.org/10.1007/BF00186600

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  • DOI: https://doi.org/10.1007/BF00186600

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