Abstract
The expression of genes encoding self-incompatibility ribonucleases (S-RNases) in Nicotiana alata were examined at both protein and RNA level for organ specificity. S-RNases recovered from stigmas and styles were indistinguishable by SDS-PAGE, chromatographic behaviour and RNase specific activity. The pistil S transcripts are heterogenous in size, the stigma transcript being shorter and more heterogenous than the transcripts in the style and ovary. RNase H analysis shows that this organ-specific difference is mainly in the length of the polyadenylate tail. By sequence analysis of cloned cDNAs we show that the transcript present in the stigma is derived from the same gene as the transcript in the style.
This is a preview of subscription content, log in via an institution to check access.
References
Anderson MA, Cornish EC, Mau S-L, Williams EG, Hoggart R, Atkinson A, Bönig I, Grego B, Simpson R, Roche PJ, Haley JD, Niall HD, Tregear GW, Coghlan JP, Clarke AE: Cloning of a cDNA for a stylar glycoprotein associated with the expression of self-incompatibility in Nicotiana alata. Nature 321: 38–44 (1986).
Brewer G, Ross J: Poly(A) shortening and degradation of the 3′ A+U rich sequences of human c-myc mRNA in a cell free system. Mol Cell Biol 8: 1697–1708 (1988).
Chen C-G, Mau S-L, Clarke AE: Nucleotide sequence and style-specific expression of a novel proline-rich protein gene from Nicotiana alata. Plant Mol Biol, in press (1993).
de Nettancourt D: Incompatibility in Angiosperms. Springer-Verlag, Berlin (1977).
Galau GA, Legocki AB, Greenway SC, Dure LS III: Cotton messenger RNA sequences exist in both polyadenylated and nonpolyadenylated forms. J Biol Chem 256: 2551–2560 (1981).
Haring V, Gray JE, McClure BA, Anderson MA, Clarke AE: Self-incompatibility: a self recognition system in plants. Science 250: 937–941 (1990).
Jahnen W, Batterham MP, Clarke AE, Moritz RL, Simpson J: Identification, isolation and N-terminal sequencing of style glycoproteins associated with self-incompatibility in Nicotiana alata. Plant Cell 1: 492–500 (1989).
Jahnen W, Lush WM, Clarke AE: Inhibition of in vitro pollen tube growth by isolated S-glycoproteins of Nicotiana alata. Plant Cell 1: 501–510 (1989).
Mau S-L: Molecular studies of gametophytic selfincompatibility. Ph.D. thesis, University of Melbourne (1991).
McClure BA, Gray JE, Anderson MA, Clarke AE: Self-incompatibility in Nicotiana alata involves degradation of pollen ribosomal RNA. Nature 347: 757–760 (1990).
McClure BA, Haring V, Ebert PR, Anderson MA, Simpson RJ, Sakiyama F, Clarke AE: Style self-incompatibility gene products of Nicotiana alata are ribonucleases. Nature 342: 955–957 (1989).
Mercer JFB, Wake SA: An analysis of the rate of metallothionein mRNA poly(A)-shortening using RNA blot hybridization. Nucl Acids Res 13: 7929–7943 (1985).
Nasrallah JB, Nishio T, Nasrallah M: The self-incompatibility genes of Brassica: Expression and use in genetic ablation of floral tissues. Annu Rev Plant Physiol Plant Mol Biol 42: 393–422 (1991).
Willing PR, Mascarenhas JP: Analysis of the complexity and diversity of mRNAs from pollen and shoots of Tradescantia. Plant Physiol 75: 865–868 (1984).
Woodward JR, Bacic A, Jahnen W, Clarke AE: N-linked glycan chains on S-allele-associated glycoproteins from Nicotiana alata. Plant Cell 1: 511–514 (1989).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
McClure, B.A., Du, H., Liu, YH. et al. S-locus products in Nicotiana alata pistils are subject to organ-specific post-transcriptional processing but not post-translational processing. Plant Mol Biol 22, 177–181 (1993). https://doi.org/10.1007/BF00039008
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00039008